a-fodrin

Monoclonal antibody to
a-FODRIN (NONERYTHROID SPECTRIN)
clone AA6 (IgG1)

Cat. No. 610013

100 µg immunoglobulin in 1 ml PBS solution containing 1.0% (w/v) BSA and 0.1% (w/v) sodium azide.

The product is for research use only. The performance characteristics of this product have not been established. Use in human clinical diagnosis is the responsibility of the user. This product should be stored
at +2 … +8 °C.

Monoclonal antibody to a-fodrin is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Chicken red blood cell membranes purified by hypotonic lysis and mechanical enucleation were used as immunogen.

Specificity
The antibody is specific to the 240 kD a-fodrin molecule of all mammalian nonerythroid cells and chicken a-spectrin. The antibody crossreacts with human, rat, chicken, rabbit and guinea pig.

Applications
Immunohistochemical stainings, western blotting and other immunoassays for cellular and tumor biology.

Guidelines for dilutions
Working dilution in western blotting is at least 1:1000.

References

1. Sormunen R. et al. (1999) J. Pathol. 187, 416-423.
   
2. Vääräniemi J. et al. (1997) Eur. J. Cell Biol. 74, 262-272.
   
3. Tuominen H. et al. (1996) Br. J. Dermatol. 135, 576-580.
   
4. Huotari V. et al. (1996) J.Cell. Physiol. 167, 121-130.
   
5. Sormunen R. et al. (1994) Am. J. Resp. Cell Mol. Biol. 11, 75-84.
   
6. Vääräniemi J. et al. (1994) Biochim. Biophys. Acta 1189, 21-30.
   
7. Sormunen R. et al. (1993) Histochem. J. 25, 678-686.
   
8. Sormunen R. et al. (1993) Lab. Invest. 68, 652-662.
   
9. Reima I. et al. (1993) Differentiation 54, 35-45.
   
10. Eskelinen S. et al. (1992) J. Cell. Physiol. 150, 122-133.
    
11. Huotari V. et al. (1992) J. Cell. Physiol. 153, 340-352.
    
12. Ylikoski J. et al. (1990) Hearing Res. 43, 199-204.