Pepsinogen II ELISA

Pepsinogen II ELISA Kit 96 wells Cat.No. 601020*

Background
Pepsinogen II (or pepsinogen C) belongs to a group of aspartic proteinase zymogens (inactive form) that are activated in the acid pH of stomach to active enzyme pepsin. Pepsinogen II (PG II) is produced by chief cells and mucus neck cells of gastric mucosa, pyloric glands in the antrum and Brunner’s glands in the proximal duodenum. Serum concentration of PG II reflects the histological status of gastric mucosa. The ratio of concentration of PGI to PGII in serum of normal subjects is about 4:1 (1). The PGI/PGII ratio decreases linearly with increasing grade of atrophic gastritis in the corpus (2, 3). The ratio is < 2.5 when the atrophic gastritis is advanced (moderate or severe) in the gastric corpus (3).

Principle of the Assay
The assay is based on a sandwich ELISA technique with specific capture antibody adsorbed on a microplate and detection antibody labelled with enzyme (HRP).

Assay Highlights

Exellent sensitivity enables measuring reliably concentrations
< 1.0 µg/l
Within-assay precision averagely 4.5 %
Between-assay precision averagely 6.8 %
Total incubation time 2.5 hours, enables fast turnaround of
sample results
Protocol is easily adapted for automated systems

References

Samloff IM. Peptic activity and peptic inhibitors. In Gitnick G, ed. Principles and practice of gastroenterology and hepatology.
Pp. 154-163. New York: Elsevier, 1988.
Varis K. Surveillance of pernicious anemia. In Scherlock P, Morson PC, Barbara L, Veronesi U, ed. Precancerous lesions of the gastrointestinal tract. Pp. 189-194. New York, Raven Press, 1983.
Varis K, Samloff IM, Ihamäki T, Siurala M. An appraisal of tests for severe atrophic gastritis in relatives of patients with pernicious anemia. Dig. Dis. Sci., 1979; 24: 187-191.

*In the USA: For research use only. Not for use in diagnostic procedures. Pending FDA clearance.